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In another preferred embodiment, the invention also provides for methods of expressing Cas9 under the control of an inducible promoter, and the constructs used .. In certain embodiments of the invention, the first and second tracr mate sequence share % identity and/or the first and second tracr sequence share %. The Quick-Load 1 kb Extend DNA ladder is a pre-mixed, ready-to-load molecular weight DNA marker containing bromophenol blue dye as a tracking dye. The DNA Ladder consists of proprietary plasmids and DNA molecules which are digested to completion with appropriate restriction enzymes to yield 13 bands suitable. 21 Oct In another preferred embodiment, the invention also provides for methods of expressing Cas9 under the control of an inducible promoter, and the [] AAV has a packaging limit of or Kb. This means that Cas9 as well as a promoter and transcription terminator have to be all fit into the same.

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The digested DNA includes fragments ranging frombase pairs.

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Your session has expired. For your security, we have logged you out. Would you like to log in again? Printed from VWR Website. NS NL A number of proprietary plasmids are digested to completion with appropriate restriction enzymes to yield 17 bands suitable for use as molecular weight standards for agarose and polyacrylamide gel electrophoresis.

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